Title: Molecular and Cellular Biologist

Location: Cambridge-MA

Duration: 6+ months

Job Description:

• We are seeking a scientist to join our Systems Immunology Lab in ***’s Inflammation & Immunology Research Unit.
• The successful candidate will be responsible for generating and processing single-cell, spatial, and functional genomics data, as well as supporting standard immunoprofiling activities such as cell culture and flow cytometry.
• Follow existing lab standard operating procedures.
• Participate in sample collection of murine and human tissues, including the preparation of tubes, interfacing with in vivo scientists to retrieve samples, isolation of primary immune cells, and standard mammalian cell culture.
• Participated in sample preparation of murine and human tissues, including tissue fixation, dissociation, sectioning, and antibody staining.
• Generate spatial and single-cell data via a number of commercial workflows, including 10x Genomics and Parse Technologies.
• Interpret quality control metrics for spatial, single-cell and cytometry experiments.

Education And Experience:

• Masters + 3 years of experience in Biology, Immunology, or a related field
• or
• Ph'D +0 yrs experience in Biology, Immunology, or a related field.

Technical Skills Requirements:

Bonus: experience with CRISPR mediated functional genomics, including electroporation of primary immune cells.

3+ years of hands-on experience with standard immunoprofiling technologies, including-

• Flow Cytometry
• Single-cell library preparation (ideally 10x Genomics or Parse Technology)
• Preparation and loading of Illumina sequencing libraries
• Bonus: generation of spatial transcriptomics data (any technology)

3+ years of experience with primary immune cells, including-

• Primary immune cell isolation
• Maintenace of immune cell lines
• Cell culture and in vitro stimulation of primary immune cells