OLIGONUCLEOTIDE CHEMIST

Location: Cambridge, MA (Onsite)

Salary: $90K to $100k base

PRIMARY RESPONSIBILITIES :

• Be responsible for the day-to-day work and management of the Oligonucleotide teams in Boston
• Design and implement innovative strategies for the synthesis, purification, and scale-up of oligonucleotides
• Utilize hands-on experience to optimize synthetic routes, purification methods, and bioconjugation protocols
• Conduct analytical characterization using HPLC, LC-MS, and other analytical methods.
• Complete the goals associated with specific oligonucleotide process optimization projects.
• Develop solutions and provide recommendations as to the appropriate course of action to technical problems associated with the preparation of synthetic oligonucleotides.
• Provides documentation and validation support in execution of synthesis and/or purification process changes.
• Performs laboratory work and documentation in accordance with regulatory standards and company guidelines.
• Mentor oligo team members, providing technical guidance and expertise in oligonucleotide chemistry

EDUCATION AND EXPERIENCE:

• Minimum of a bachelor’s degree in Biochemistry, Chemistry, Molecular Biology or related discipline.
• Minimum of 5 years of industrial or academic experience in the synthesis and purification of oligonucleotides.
• Excellent understanding of the basic structure and function of DNA and RNA, including but not limited to advanced applications such as gene cloning and NGS preparation.

SKILL REQUIREMENTS:

Competence & Skills

• Experienced in high-throughput oligonucleotides synthesis, proficient in the operation of the 192&768 synthesizer, and carrying out simple instrument troubleshooting and repair.
• Proficient in multiple analytical and purification techniques for DNA/RNA oligonucleotides to ensure that the purity and quality of oligonucleotides meet experimental requirements.
• Experienced in the application of LC-MS technology for accurate qualitative and quantitative analysis of nucleotides.
• Knowledge of commonly used biomolecular analysis methods (e.g. qPCR, DNA sequencing) or relevant practical experience.
• Knowledge of the downstream application of oligonucleotides, like gene synthesis, molecular cloning, NGS, etc.

Professional Attitude

• Good scientific thinking and innovation ability, able to independently design experimental programs and promote project progress.
• Good teamwork spirit, able to communicate effectively with other researchers and solve research problems together.
• Strong willingness to learn and ability to quickly adapt to new technologies and methods.
• Welcome the challenge and extra responsibilities.
• With clear career aspiration and willing to make impact to the environment

WORKING RELATIONSHIPS

• Report to: Oligo Team Leader
• You will be working with internal oligo team, as well as downstream gene synthesis, molecular cloning and NGS team at Boston